4XSG
The complex structure of C3cer exoenzyme and GTP bound RhoA (NADH-free state)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 32 |
| Unit cell lengths | 50.745, 50.745, 135.871 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.290 - 1.800 |
| R-factor | 0.17714 |
| Rwork | 0.175 |
| R-free | 0.22274 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a2b 3bw8 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.350 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (1.9) |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.800 |
| Number of reflections | 36314 |
| <I/σ(I)> | 39.3 |
| Completeness [%] | 100.0 |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 100 mM MES (pH 6.4), 20% PEG1500 |
