4XRN

Pilz domain with c-di-gmp of a protein from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.989
Spacegroup name	P 41 21 2
Unit cell lengths	75.526, 75.526, 195.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.859 - 2.000
R-factor	0.2328
Rwork	0.231
R-free	0.27380
RMSD bond length	0.008
RMSD bond angle	1.496
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: dev_1894))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.000
Number of reflections	39278
<I/σ(I)>	74.585
Completeness [%]	100.0
Redundancy	20

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	Sodium chloride, Imidazole, Hydrochloric acid, Zinc acetate