4XQM

Crystal structure of the MRH domain of Glucosidase II beta bound to mannose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	98
Detector technology	IMAGE PLATE
Collection date	2013-07-01
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	I 4
Unit cell lengths	57.571, 57.571, 58.321
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.161 - 1.625
R-factor	0.1703
Rwork	0.167
R-free	0.19690
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.396
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELX
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.650
High resolution limit [Å]	1.620	4.400	1.620
Rmerge	0.064	0.030	0.395
Total number of observations	153936
Number of reflections	12000
<I/σ(I)>	14.5
Completeness [%]	99.0	99.7	79.6
Redundancy	12.8	14.3	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.25	292	10mg/ml protein in 20mM Tris pH 7.5, 150mM NaCl, 100mM mannose mixed 1:1 with 100 mM triethanolamine HCl pH 8.25, 200 mM potassium glutamate, 28% PEG 4000