4XP4

X-ray structure of Drosophila dopamine transporter in complex with cocaine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-09-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	97.063, 140.727, 166.688
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.880 - 2.800
Rwork	0.236
R-free	0.26600
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.880	2.970
High resolution limit [Å]	2.800	2.800
Rmerge		1.805
Number of reflections	107985
<I/σ(I)>		0.94
Completeness [%]	98.9	95.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	277	PEG 400 (37%), NaMES (0.1M)