4XOI

Structure of hsAnillin bound with RhoA(Q63L) at 2.1 Angstroms resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	103
Detector technology	CCD
Collection date	2012-09-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.987
Spacegroup name	P 1
Unit cell lengths	54.311, 52.434, 103.101
Unit cell angles	77.87, 74.14, 85.73

Refinement procedure

Resolution	29.688 - 2.092
R-factor	0.1737
Rwork	0.173
R-free	0.20610
RMSD bond length	0.007
RMSD bond angle	1.048
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall
Low resolution limit [Å]	29.688
High resolution limit [Å]	2.092
Number of reflections	61564
<I/σ(I)>	3
Completeness [%]	98.1
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	12% PEG 3,350, 3% PEG 10000, 0.2 M sodium malonate, 5 mM DTT