4XOI
Structure of hsAnillin bound with RhoA(Q63L) at 2.1 Angstroms resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2012-09-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 |
| Unit cell lengths | 54.311, 52.434, 103.101 |
| Unit cell angles | 77.87, 74.14, 85.73 |
Refinement procedure
| Resolution | 29.688 - 2.092 |
| R-factor | 0.1737 |
| Rwork | 0.173 |
| R-free | 0.20610 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.048 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 29.688 |
| High resolution limit [Å] | 2.092 |
| Number of reflections | 61564 |
| <I/σ(I)> | 3 |
| Completeness [%] | 98.1 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 12% PEG 3,350, 3% PEG 10000, 0.2 M sodium malonate, 5 mM DTT |
