4XO1
crystal structure of Se-Met GnsA with double mutations
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 3W1A |
| Synchrotron site | BSRF |
| Beamline | 3W1A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-05 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 38.074, 42.539, 72.782 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.370 - 1.802 |
| R-factor | 0.196 |
| Rwork | 0.195 |
| R-free | 0.22320 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.220 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.060 | 0.414 |
| Number of reflections | 10526 | |
| <I/σ(I)> | 60.3 | 6.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 14.1 | 14.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.1M BIS-TRIS pH 5.5, 25%(w/v) Polyethylene glycol 3,350 |
