4XNG
Central Domain of Mycoplasma Genitalium Terminal Organelle protein MG491
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-31 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.979154 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 98.270, 108.420, 62.190 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.000 |
| R-factor | 0.227 |
| Rwork | 0.226 |
| R-free | 0.24910 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.405 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHELXDE |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.199 | |
| Number of reflections | 13879 | |
| <I/σ(I)> | 10.6 | 2.4 |
| Completeness [%] | 95.3 | 100 |
| Redundancy | 19.5 | 20.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | PEG3350, Li2SO4, Tris |
