4XMR

Crystal structure of the sensory domain of the Campylobacter jejuni chemoreceptor Tlp3 (CcmL) with isoleucine bound.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-09-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.95370
Spacegroup name	P 1 21 1
Unit cell lengths	42.620, 138.010, 49.020
Unit cell angles	90.00, 94.33, 90.00

Refinement procedure

Resolution	26.997 - 1.300
R-factor	0.1421
Rwork	0.141
R-free	0.17290
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.097
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.890	39.890	1.320
High resolution limit [Å]	1.300	7.120	1.300
Rmerge	0.053	0.048	0.346
Rpim	0.033	0.034	0.261
Total number of observations	441329	1065	10462
Number of reflections	128260
<I/σ(I)>	12.2	21.4	2.1
Completeness [%]	92.9	42.7	59.2
Redundancy	3.4	2.8	2.6
CC(1/2)	0.997	0.984	0.830

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	PEG3350, Ammonium sulfate, Isoleucine