4XMH
Crystal structure of nitrophorin 7 from Rhodnius prolixus at pH 7.8 complexed with Gly-Gly-Gly
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-12-20 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 38.374, 66.929, 38.885 |
Unit cell angles | 90.00, 116.79, 90.00 |
Refinement procedure
Resolution | 34.710 - 1.290 |
R-factor | 0.1559 |
Rwork | 0.154 |
R-free | 0.18220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3mvf |
RMSD bond length | 0.006 |
RMSD bond angle | 1.067 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: 2011_08_24_1020)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.710 | 1.370 |
High resolution limit [Å] | 1.290 | 1.290 |
Rmerge | 0.024 | 0.290 |
Number of reflections | 41095 | |
<I/σ(I)> | 21.4 | 2.3 |
Completeness [%] | 92.8 | 64.7 |
Redundancy | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 25%(w/v) PEG 3350, 0.1 M bis-Tris propane pH 7.8, 0.25%(w/v) Gly-Gly, 0.25%(w/v) Gly-Gly-Gly, 0.25%(w/v) Gly- Gly-Gly-Gly, 0.25%(w/v) pentaglycine, 0.02 M HEPES sodium pH 6.8. |