4XLG
C. glabrata Slx1 in complex with Slx4CCD.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-09 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97626 |
| Spacegroup name | P 63 |
| Unit cell lengths | 135.790, 135.790, 56.710 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 1.780 |
| R-factor | 0.1589 |
| Rwork | 0.158 |
| R-free | 0.18180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xm5 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.461 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.840 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.081 | 1.250 |
| Number of reflections | 57362 | |
| <I/σ(I)> | 16.3 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.2 | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M magnesium chloride hexahydrate, 0.1 M Bis Tris (pH 6.5), 25% (v/v) PEG 3350 |
