4XKK
Crystal structure of N-terminal domain of Hsp90 from Dictyostelium discoideum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU ULTRAX 18 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-10-12 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.240, 44.860, 60.249 |
| Unit cell angles | 90.00, 107.53, 90.00 |
Refinement procedure
| Resolution | 28.680 - 2.700 |
| R-factor | 0.22036 |
| Rwork | 0.218 |
| R-free | 0.27164 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yes |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.129 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.310 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.079 | 0.372 |
| Number of reflections | 5876 | |
| <I/σ(I)> | 19.4 | 5.2 |
| Completeness [%] | 99.6 | 99.2 |
| Redundancy | 5.6 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | 0.1M Hepes, PEG 3350 |
