4XKF
Crystal structure of hemagglutinin from Taiwan (2013) H6N1 influenza virus in complex with LSTa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.03320 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 185.735, 99.271, 133.437 |
| Unit cell angles | 90.00, 126.45, 90.00 |
Refinement procedure
| Resolution | 49.636 - 2.446 |
| R-factor | 0.2129 |
| Rwork | 0.211 |
| R-free | 0.25280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xkd |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.567 |
| Data reduction software | HKL-2000 (705b) |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.490 |
| High resolution limit [Å] | 2.446 | 6.650 | 2.446 |
| Rmerge | 0.103 | 0.055 | 0.448 |
| Rmeas | 0.121 | 0.065 | 0.539 |
| Rpim | 0.063 | 0.032 | 0.294 |
| Total number of observations | 249206 | ||
| Number of reflections | 71296 | ||
| <I/σ(I)> | 6.7 | ||
| Completeness [%] | 99.1 | 99.8 | 93 |
| Redundancy | 3.5 | 3.6 | 2.9 |
| CC(1/2) | 0.995 | 0.776 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293.15 | 10mM NiCl2, 0.1M Tris pH 8.5, 20% (w/v) MPEG 2000 and 20% glycerol |
