4XKC
Crystal Structure of GENOMES UNCOUPLED 4 (GUN4) in Complex with Magnesium Deuteroporphyrin IX
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-11-14 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 32.849, 64.026, 106.144 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.013 - 2.000 |
R-factor | 0.1841 |
Rwork | 0.182 |
R-free | 0.22890 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.191 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | HKL-2000 |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.000 |
Number of reflections | 16064 |
<I/σ(I)> | 18.7 |
Completeness [%] | 99.9 |
Redundancy | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | sodium cacodylate, sodium chloride, PEG 3350 |