4XKA
N-terminal domain of Hsp90 from Dictyostelium discoideum in hexagonal form with glycerol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-04 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.95372 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 156.320, 156.320, 45.870 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 135.380 - 2.000 |
| R-factor | 0.22962 |
| Rwork | 0.228 |
| R-free | 0.25691 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xcj |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.525 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 135.380 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.079 | 0.739 |
| Number of reflections | 22849 | |
| <I/σ(I)> | 23.4 | 4.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 18.1 | 18.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 290 | 0.1M Tris, Ammonium sulphate, glycerol |
