4XJY
Periplasmic repressor protein YfiR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-08-14 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.97910 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.691, 68.073, 71.989 |
| Unit cell angles | 90.00, 111.42, 90.00 |
Refinement procedure
| Resolution | 47.756 - 1.800 |
| R-factor | 0.1753 |
| Rwork | 0.173 |
| R-free | 0.21290 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.225 |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | SHELX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.756 | 47.756 | 1.900 |
| High resolution limit [Å] | 1.799 | 5.690 | 1.800 |
| Rmerge | 0.029 | 0.170 | |
| Rmeas | 0.091 | ||
| Rpim | 0.034 | 0.024 | 0.122 |
| Total number of observations | 346155 | 13204 | 24476 |
| Number of reflections | 54499 | ||
| <I/σ(I)> | 16.8 | 38.4 | 5.1 |
| Completeness [%] | 93.5 | 98.2 | 72.8 |
| Redundancy | 6.4 | 7 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.8 M Potassium sodium tartrate tetrahydrate, 0.1 M Tris-HCl pH 8.0, 0.5 % Polyethylene glycol monoethyl ether 5000 |
