4XIY
Crystal structure of ketol-acid reductoisomerase from Azotobacter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-05-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0000, 1.7448 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 185.050, 185.050, 185.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.790 - 2.500 |
| R-factor | 0.19646 |
| Rwork | 0.194 |
| R-free | 0.24377 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1np3 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.720 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.760 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.092 | 0.513 |
| Number of reflections | 72509 | |
| <I/σ(I)> | 10.9 | 2.6 |
| Completeness [%] | 99.5 | 99.9 |
| Redundancy | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 0.25 M NaCl, 28 % PEG3350 and 0.1 M Bis-Tris, pH 5.5, strictly anaerobic |
