4XIM

PROTEIN ENGINEERING OF XYLOSE (GLUCOSE) ISOMERASE FROM ACTINOPLANES MISSOURIENSIS. 1. CRYSTALLOGRAPHY AND SITE-DIRECTED MUTAGENESIS OF METAL BINDING SITES

Experimental procedure

Spacegroup name	P 32 2 1
Unit cell lengths	143.450, 143.450, 231.500
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	? - 2.300
R-factor	0.158
RMSD bond length	0.011
RMSD bond angle	0.039
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.300 *
Rmerge	0.062 *
Total number of observations	225965 *
Number of reflections	108255 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	25 (mg/ml)
2	1	drop	ammonium sulfate	1 (M)
3	1	drop	sodium phosphate	0.1 (M)
4	1	reservoir	ammonium sulfate	1.2 (M)