4XI8
Crystal Structure of the FIC domain of Bep5 protein (VirB-translocated Bartonella effector protein) from Bartonella clarridgeiae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-07-02 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97857 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 99.270, 122.860, 143.710 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.021 - 2.950 |
R-factor | 0.2373 |
Rwork | 0.235 |
R-free | 0.27890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vza |
RMSD bond length | 0.002 |
RMSD bond angle | 0.539 |
Data scaling software | XSCALE |
Phasing software | BALBES |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.030 | |
High resolution limit [Å] | 2.950 | 13.190 | 2.950 |
Rmerge | 0.075 | 0.029 | 0.516 |
Rmeas | 0.082 | 0.033 | 0.562 |
Total number of observations | 229118 | ||
Number of reflections | 37608 | 436 | 2729 |
<I/σ(I)> | 17.35 | 34.1 | 3.7 |
Completeness [%] | 99.7 | 88.1 | 100 |
Redundancy | 6.1 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 19.9 mg/mL protein, 1:1 with Wiz3/4(g5): 20% PEG3350, 0.1 M sodium citrate/citric acid, pH 4.0, 0.2 M sodium citrate tribasic |