4XHP
Bacillus thuringiensis ParM hybrid protein with ADP, containing two ParM mutants
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.306, 97.275, 87.898 |
| Unit cell angles | 90.00, 98.82, 90.00 |
Refinement procedure
| Resolution | 27.750 - 3.200 |
| R-factor | 0.2349 |
| Rwork | 0.229 |
| R-free | 0.28710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.307 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.5.5) |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 6.870 | 3.200 |
| Rmerge | 0.062 | 0.021 | 0.313 |
| Total number of observations | 46526 | ||
| Number of reflections | 15010 | ||
| <I/σ(I)> | 10.1 | ||
| Completeness [%] | 97.5 | 96.5 | 96.1 |
| Redundancy | 3.1 | 3.2 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 288 | 0.1 M sodium acetate, pH 5.0, 5% poly-gamma-glutamic acid polymer, 30% PEG 400 |
