4XHO

Bacillus thuringiensis ParM with ATP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-10-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.000
Spacegroup name	P 2 21 21
Unit cell lengths	55.830, 73.156, 113.726
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.825 - 2.650
R-factor	0.2099
Rwork	0.203
R-free	0.27120
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.587
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER (2.3.0)
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.700
High resolution limit [Å]	2.650	7.160	2.650
Rmerge	0.057	0.031	0.495
Total number of observations	102248
Number of reflections	14327
<I/σ(I)>	15.4
Completeness [%]	100.0	99.5	100
Redundancy	7.1	6.4	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	288	0.1 M Bis-Tris, pH 6.5, 0.2 M ammonium acetate, 25% PEG 3350