4XHI

Crystal structure of native Thosea asigna virus RNA-dependent RNA polymerase (RdRP) at 2.15 Angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.98
Spacegroup name	P 21 21 2
Unit cell lengths	134.875, 150.825, 100.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.606 - 2.150
R-factor	0.1773
Rwork	0.175
R-free	0.21580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xha
RMSD bond length	0.002
RMSD bond angle	0.641
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.000	2.270
High resolution limit [Å]	2.150	2.150
Rmerge	0.120	0.294
Number of reflections	109020
<I/σ(I)>	9	4.1
Completeness [%]	97.5	95.6
Redundancy	4.6	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	12% PEG 8K, 750 mM lithium(III) sulfate