4XGU
Structure of C. elegans PCH-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 98 |
| Detector technology | PIXEL |
| Collection date | 2014-03-03 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 126.710, 240.970, 197.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.360 - 2.301 |
| R-factor | 0.2302 |
| Rwork | 0.228 |
| R-free | 0.26400 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.728 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | SHELX |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.330 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Rmerge | 0.098 | 3.143 |
| Number of reflections | 134133 | |
| <I/σ(I)> | 17.9 | 0.8 |
| Completeness [%] | 99.5 | 90.8 |
| Redundancy | 13.5 | 10.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 100 mM sodium citrate pH 5.6, 200 mM ammonium sulfate, 15% PEG 3350 |
