4XGU
Structure of C. elegans PCH-2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 98 |
Detector technology | PIXEL |
Collection date | 2014-03-03 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 126.710, 240.970, 197.950 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.360 - 2.301 |
R-factor | 0.2302 |
Rwork | 0.228 |
R-free | 0.26400 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.728 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | SHELX |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.330 |
High resolution limit [Å] | 2.290 | 2.290 |
Rmerge | 0.098 | 3.143 |
Number of reflections | 134133 | |
<I/σ(I)> | 17.9 | 0.8 |
Completeness [%] | 99.5 | 90.8 |
Redundancy | 13.5 | 10.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 100 mM sodium citrate pH 5.6, 200 mM ammonium sulfate, 15% PEG 3350 |