4XG4
Crystal structure of an inhibitor-bound Syk
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.219, 85.056, 41.749 |
| Unit cell angles | 90.00, 98.80, 90.00 |
Refinement procedure
| Resolution | 42.530 - 2.300 |
| R-factor | 0.19522 |
| Rwork | 0.194 |
| R-free | 0.22843 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gx2 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.202 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 12408 | |
| <I/σ(I)> | 24.13 | |
| Completeness [%] | 98.3 | |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 278 | 10~20%(v/v) PEG3350, 100 mM Tris-HCl |
