4XFU
Structure of IL-18 SER Mutant V
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.688, 52.032, 123.368 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.002 - 2.850 |
R-factor | 0.2494 |
Rwork | 0.244 |
R-free | 0.28680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3f62 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.119 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.850 | 2.850 |
Number of reflections | 6953 | |
<I/σ(I)> | 9 | 1.6 |
Completeness [%] | 98.3 | 99.9 |
Redundancy | 5.7 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 35% PEG 3350, 0.1M Tris, 0.1M Sodium Acetate |