4XFO

Structure of an amyloid-forming segment TAVVTN from human Transthyretin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-12-09
Detector	PSI PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 1
Unit cell lengths	4.750, 10.660, 16.390
Unit cell angles	77.64, 87.81, 77.61

Refinement procedure

Resolution	16.010 - 1.350
R-factor	0.135
Rwork	0.134
R-free	0.14780
RMSD bond length	0.009
RMSD bond angle	1.270
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	16.010
High resolution limit [Å]	1.350
Number of reflections	629
<I/σ(I)>	6.93
Completeness [%]	92.6
Redundancy	21.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	0.2 M Ammonium sulfate, 0.1 M BIS-TRIS pH 5.5, 25% w/v Polyethylene glycol 3,350, 25% Glycerol