4XFH
cysteine dioxygenase variant - Y157F at pH 6.2 with cysteine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-02-13 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.977 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 57.600, 57.600, 122.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.023 - 1.350 |
R-factor | 0.1651 |
Rwork | 0.162 |
R-free | 0.19280 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 4ieu |
RMSD bond length | 0.011 |
RMSD bond angle | 1.258 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.000 | 1.370 |
High resolution limit [Å] | 1.350 | 1.350 |
Number of reflections | 45159 | |
<I/σ(I)> | 17.8 | 0.7 |
Completeness [%] | 97.8 | 97.8 |
Redundancy | 23 | 8.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M tri-sodium citrate pH=5.6, 24% PEG 4000, 0.15M ammonium acetate. 1:1 drop ratio with microseeding.; Soak solution at pH 8.0 |