4XE7
Bacillus thuringiensis ParM in apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.248, 116.430, 52.171 |
| Unit cell angles | 90.00, 115.73, 90.00 |
Refinement procedure
| Resolution | 25.319 - 2.000 |
| R-factor | 0.1885 |
| Rwork | 0.186 |
| R-free | 0.23330 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.147 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.3.0) |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 5.430 | 2.000 |
| Rmerge | 0.096 | 0.090 | 0.317 |
| Total number of observations | 136335 | ||
| Number of reflections | 33791 | ||
| <I/σ(I)> | 12.8 | ||
| Completeness [%] | 94.7 | 98 | 74.7 |
| Redundancy | 4 | 4.2 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 288 | 0.2 M potassium citrate, 20% PEG 3350 |
