4XD9

Structure of Rpf2-Rrs1 complex involved in ribosome biogenesis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2012-07-04
Detector	RAYONIX MX225HE
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	54.163, 123.594, 133.817
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.098 - 2.350
R-factor	0.1945
Rwork	0.192
R-free	0.23490
RMSD bond length	0.003
RMSD bond angle	0.910
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.350
Number of reflections	38131
<I/σ(I)>	14.51
Completeness [%]	99.4
Redundancy	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG 6000, HEPES