4XD0
X-ray structure of the N-formyltransferase QdtF from Providencia alcalifaciens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-05-13 |
Detector | Bruker Platinum 135 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 78.134, 78.134, 151.424 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.970 - 1.800 |
R-factor | 0.20737 |
Rwork | 0.204 |
R-free | 0.26455 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4xcz |
RMSD bond length | 0.010 |
RMSD bond angle | 1.777 |
Data reduction software | SAINT |
Data scaling software | SADABS |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.850 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.081 | 0.385 |
Number of reflections | 42223 | |
<I/σ(I)> | 11.9 | 2.2 |
Completeness [%] | 95.4 | 89.7 |
Redundancy | 6.2 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 5-7 % PEG 8000, 100 mM HEPPS, 200 mM sodium chloride, 200 mM potassium chloride, 5 mM TDP, 5 mM N5-THF |