4XCQ
Clostridium botulinum phage c-st TubZ (C-terminal tail truncated protein)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98011 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 104.970, 86.342, 44.734 |
| Unit cell angles | 90.00, 92.49, 90.00 |
Refinement procedure
| Resolution | 44.690 - 2.390 |
| R-factor | 0.1981 |
| Rwork | 0.196 |
| R-free | 0.23950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3v3t |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.800 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.690 | 2.530 |
| High resolution limit [Å] | 2.390 | 2.390 |
| Number of reflections | 15849 | |
| <I/σ(I)> | 7.7 | 0.93 |
| Completeness [%] | 99.4 | 96.7 |
| Redundancy | 6.6 | |
| CC(1/2) | 0.989 | 0.462 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293.15 | sodium citrate, sodium cloride, PEG 4000 |
