4XCJ
N-terminal domain of Hsp90 from Dictyostelium discoideum in complex with ADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-23 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97625 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 41.840, 46.650, 59.620 |
Unit cell angles | 90.00, 107.39, 90.00 |
Refinement procedure
Resolution | 56.890 - 1.750 |
R-factor | 0.20363 |
Rwork | 0.202 |
R-free | 0.23753 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yes |
RMSD bond length | 0.007 |
RMSD bond angle | 1.202 |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.890 | 1.840 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.082 | 0.499 |
Number of reflections | 22298 | |
<I/σ(I)> | 14.7 | 3.8 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.4 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | 0.1M TRIS, PEG3350 |