4XCI
Crystal structure of a hexadecameric TF55 complex from S. solfataricus, crystal form II
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-07-11 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.95370 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 148.420, 148.420, 304.670 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.830 | 48.830 | 3.150 |
High resolution limit [Å] | 3.000 | 9.950 | 3.000 |
Rmerge | 0.381 | 0.039 | 4.912 |
Rpim | 0.102 | 0.011 | 1.307 |
Total number of observations | 507888 | 13969 | 67858 |
Number of reflections | 34477 | ||
<I/σ(I)> | 6.8 | 42.2 | 0.6 |
Completeness [%] | 100.0 | 99.2 | 100 |
Redundancy | 14.7 | 13.2 | 15 |
CC(1/2) | 0.997 | 0.998 | 0.408 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.9 | 293 | Tris-HCl, PEG 2000, 2-propanol, strontium chloride, TMAO |