4XBI
Structure Of A Malarial Protein Involved in Proteostasis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-16 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 65 |
| Unit cell lengths | 127.530, 127.530, 92.570 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 70.946 - 2.013 |
| R-factor | 0.1685 |
| Rwork | 0.167 |
| R-free | 0.20160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b3p 4IOD and 4IRF |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.035 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.946 | 2.070 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rmerge | 0.079 | 0.488 |
| Number of reflections | 55948 | |
| <I/σ(I)> | 10.94 | 2.68 |
| Completeness [%] | 98.6 | 90.4 |
| Redundancy | 5 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 2 M ammonium sulphate 100 mM Hepes pH=7 protein at 13-15 mg/ml |
