4XB5
Structure of orange carotenoid protein binding canthaxanthin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 82.894, 82.894, 87.201 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.434 - 1.900 |
| R-factor | 0.1828 |
| Rwork | 0.181 |
| R-free | 0.20760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Truncated form of 3MG1 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.903 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1690)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.434 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.017 | 0.363 |
| Number of reflections | 27757 | |
| <I/σ(I)> | 23.9 | 2.09 |
| Completeness [%] | 99.9 | 99.38 |
| Redundancy | 20.7 | 2.03 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 100 mM sodium acetate pH 4.5, 10% poly-ethylene glycol 20,000, 3% glycerol |
