4XB3

Structure of dextran glucosidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9800
Spacegroup name	P 21 21 21
Unit cell lengths	72.151, 82.550, 103.559
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.108 - 2.093
R-factor	0.1813
Rwork	0.179
R-free	0.21900
RMSD bond length	0.003
RMSD bond angle	0.704
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.220
High resolution limit [Å]	2.090	2.090
Number of reflections	36936
<I/σ(I)>	14.81	4.45
Completeness [%]	99.4	97.4
Redundancy	5.84	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	293	100mM Sodium HEPES, 200mM CaCl2, 180mg/ml PEG6000