4XB0

Structure of the Plk2 polo-box domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	93
Detector technology	CCD
Collection date	2014-10-02
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97949
Spacegroup name	I 2 3
Unit cell lengths	152.292, 152.292, 152.292
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.922 - 2.701
R-factor	0.2289
Rwork	0.225
R-free	0.26270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	the polo-box domain of Plk1
RMSD bond length	0.004
RMSD bond angle	1.048
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.700
Number of reflections	16508
<I/σ(I)>	48.1
Completeness [%]	99.6
Redundancy	22.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	1.26 M sodium phosphate, 0.14 M potassium phosphate