4XA4
Crystal Structure of the coiled-coil surrounding Skip 3 of MYH7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.548, 90.195, 102.717 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.542 - 2.327 |
| R-factor | 0.2188 |
| Rwork | 0.216 |
| R-free | 0.27380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ik9 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.087 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.370 |
| High resolution limit [Å] | 2.327 | 2.327 |
| Rmerge | 0.060 | 0.310 |
| Number of reflections | 22289 | |
| <I/σ(I)> | 23.4 | 8.5 |
| Completeness [%] | 99.0 | 99.9 |
| Redundancy | 7.5 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 30% (w/v) polyethylene glycol 1500, 250 mM tetramethylammonium chloride, 100 mM 3-[4-(2-Hydroxyethyl)-1-piperazinyl]propanesulfonic acid (HEPPS) |
