4XA1

Crystal Structure of the coiled-coil surrounding Skip 1 of MYH7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 1
Unit cell lengths	56.260, 59.321, 67.721
Unit cell angles	96.05, 110.01, 92.89

Refinement procedure

Resolution	34.861 - 3.200
R-factor	0.2371
Rwork	0.234
R-free	0.28510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1n04 1yib
RMSD bond length	0.013
RMSD bond angle	1.605
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.260
High resolution limit [Å]	3.200	3.200
Rmerge	0.030	0.070
Number of reflections	13198
<I/σ(I)>	12.6	7.9
Completeness [%]	97.0	98.7
Redundancy	2	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.6	298	20% (w/v) polyethylene glycol methyl ether 2000, 20 mM SrCl2, 100 mM HEPES pH 7.6, 5% pentaerythritol ethoxylate (17/8 PO/OH) 797, 0.5% 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS)