4X8N

Crystal structure of Ash2L SPRY domain in complex with phosphorylated RbBP5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	Cu FINE FOCUS
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-02-04
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 2 21 21
Unit cell lengths	38.279, 51.542, 110.201
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.900 - 2.100
R-factor	0.2165
Rwork	0.213
R-free	0.27690
RMSD bond length	0.007
RMSD bond angle	1.039
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall
Low resolution limit [Å]	29.900
High resolution limit [Å]	2.100
Number of reflections	14095
<I/σ(I)>	11.1
Completeness [%]	98.6
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	291	0.2 M magnesium chloride hexahydrate, 0.1 M BIS-TRIS, pH 5.5, 25% w/v polyethylene glycol