4X85
Crystal Structure of lipase from Geobacillus stearothermophilus T6 methanol stable variant H86Y/A269T/R374W
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-06-10 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.56 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.696, 71.391, 113.564 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.848 - 2.192 |
| R-factor | 0.2026 |
| Rwork | 0.201 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.291 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (2.3.0) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 2.190 | 2.192 |
| Rmerge | 0.080 | 0.296 |
| Number of reflections | 69491 | |
| <I/σ(I)> | 14.3 | 4.8 |
| Completeness [%] | 93.8 | 92.9 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2M sodium citrate , 25% PEG3350 |
