4X83

Crystal structure of Dscam1 isoform 7.44, N-terminal four Ig domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979150
Spacegroup name	P 1
Unit cell lengths	61.734, 88.248, 94.310
Unit cell angles	98.18, 98.79, 90.11

Refinement procedure

Resolution	49.444 - 1.902
R-factor	0.1936
Rwork	0.193
R-free	0.23500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3dmk
RMSD bond length	0.008
RMSD bond angle	1.189
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	49.444
High resolution limit [Å]	1.902
Number of reflections	148198
<I/σ(I)>	9.64
Completeness [%]	93.5
Redundancy	2.16

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.2M ammonium chloride 17%(w/v) polyethylene glycol 3350