4X83
Crystal structure of Dscam1 isoform 7.44, N-terminal four Ig domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979150 |
| Spacegroup name | P 1 |
| Unit cell lengths | 61.734, 88.248, 94.310 |
| Unit cell angles | 98.18, 98.79, 90.11 |
Refinement procedure
| Resolution | 49.444 - 1.902 |
| R-factor | 0.1936 |
| Rwork | 0.193 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dmk |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.189 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 49.444 |
| High resolution limit [Å] | 1.902 |
| Number of reflections | 148198 |
| <I/σ(I)> | 9.64 |
| Completeness [%] | 93.5 |
| Redundancy | 2.16 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.2M ammonium chloride 17%(w/v) polyethylene glycol 3350 |
