4X6Q
An Isoform-specific Myristylation Switch Targets RIIb PKA Holoenzymes to Membranes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2013-07-04 |
| Detector | ADSC QUANTUM 1 |
| Wavelength(s) | 1 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 152.714, 214.039, 61.872 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.821 - 2.520 |
| R-factor | 0.2014 |
| Rwork | 0.198 |
| R-free | 0.26140 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.348 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 44.000 |
| High resolution limit [Å] | 2.520 |
| Number of reflections | 31571 |
| <I/σ(I)> | 24 |
| Completeness [%] | 95.3 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 298 | The RIIb(R230K)2: myrC(K7C)2 holoenzyme was concentrated to 12 mg/mL. The crystal used for structure determination was obtained from a 3 uL drop containing 1:1 protein to well solution with the well solution containing 10% PEG8000, 8% ethylene glycol, 20mM MES, at pH5.8. |
