4X5L
Crystal structure of Dscam1 Ig7 domain, isoform 9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979104 |
| Spacegroup name | P 2 2 2 |
| Unit cell lengths | 45.527, 117.370, 117.333 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.527 - 2.374 |
| R-factor | 0.2214 |
| Rwork | 0.215 |
| R-free | 0.24720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dmk |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.750 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.370 |
| Number of reflections | 26120 |
| <I/σ(I)> | 21.8 |
| Completeness [%] | 99.5 |
| Redundancy | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 0.1 M HEPES PH 7.5, 0.7 M sodium phosphate monobasic, 0.7 M potassium phosphate monobasic |
