4X5K

Human NAA50 complex with coenzyme A and an acetylated peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2012-11-20
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	44.626, 53.193, 67.586
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.524 - 2.494
R-factor	0.2046
Rwork	0.202
R-free	0.25520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ob0
RMSD bond length	0.010
RMSD bond angle	1.357
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	MOLREP
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.490
High resolution limit [Å]	2.390
Number of reflections	5836
<I/σ(I)>	11.27
Completeness [%]	91.2
Redundancy	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.2 M sodium formate, 20% PEG 3350