4X4Q

Crystal structure of the A.fulgidus CCA-adding enzyme in complex with a G70A arginyl-tRNA minihelix ending in CCAC and CTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-02-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.1
Spacegroup name	P 21 21 2
Unit cell lengths	111.484, 215.582, 58.469
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.513 - 3.150
R-factor	0.2285
Rwork	0.226
R-free	0.28410
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.744
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.200
High resolution limit [Å]	3.150	8.540	3.150
Rmerge	0.095	0.034	0.349
Total number of observations	113218
Number of reflections	25262
<I/σ(I)>	6.4
Completeness [%]	98.5	92.3	98.4
Redundancy	4.5	4.3	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290	16.8% PEG3350, 0.25% NaK tartrate