4X4K
Structure of laccase from Botrytis aclada with full copper content
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | KURCHATOV SNC BEAMLINE K4.4 |
| Synchrotron site | KURCHATOV SNC |
| Beamline | K4.4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-15 |
| Detector | RAYONIX SX-165mm |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 69.400, 113.100, 79.600 |
| Unit cell angles | 90.00, 109.00, 90.00 |
Refinement procedure
| Resolution | 28.400 - 2.300 |
| R-factor | 0.1837 |
| Rwork | 0.181 |
| R-free | 0.23297 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3sqr |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.623 |
| Data reduction software | XDS (VERSION January 10, 2014) |
| Data scaling software | XSCALE (VERSION January 10, 2014) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.440 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 25106 | |
| <I/σ(I)> | 16.3 | 2.3 |
| Completeness [%] | 96.7 | 94.6 |
| Redundancy | 3.25 | 3.11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 1.8 M ammonium sulfate |
