4X4D

RADIATION DAMAGE TO THE NUCLEOPROTEIN COMPLEX C.Esp1396I: DOSE (DWD) 10.3 MGy

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2007-09-29
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9322
Spacegroup name	P 65
Unit cell lengths	104.400, 104.400, 139.160
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.038 - 2.800
R-factor	0.2371
Rwork	0.235
R-free	0.28240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3clc
RMSD bond length	0.011
RMSD bond angle	1.338
Data reduction software	MOSFLM
Data scaling software	Aimless (0.2.8)
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	69.600	69.600	2.950
High resolution limit [Å]	2.800	8.850	2.800
Rmerge	0.052	0.021	0.450
Rpim	0.023	0.011	0.197
Total number of observations	128743	3709	19229
Number of reflections	21198
<I/σ(I)>	22.4	51.4	4.2
Completeness [%]	99.9	96.2	100
Redundancy	6.1	5.5	6.2
CC(1/2)	0.997	0.989	0.912

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293.15	MES, MPD, MgCl2