4X3A
gallus interleukin-1 beta mutant - R140A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.773, 37.963, 104.319 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.090 - 1.750 |
| R-factor | 0.22279 |
| Rwork | 0.220 |
| R-free | 0.26824 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wry |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.883 |
| Data scaling software | DENZO (HKL-2000) |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 26.090 |
| High resolution limit [Å] | 1.750 |
| Number of reflections | 15110 |
| <I/σ(I)> | 32.5 |
| Completeness [%] | 98.1 |
| Redundancy | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.15 M (NH4)2SO4, 0.1 M Tris, 0.2 M LiSO4 |
