4WZX
ULK3 regulates cytokinetic abscission by phosphorylating ESCRT-III proteins
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-06-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 79.117, 79.117, 96.620 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.558 - 1.386 |
| R-factor | 0.1537 |
| Rwork | 0.151 |
| R-free | 0.18000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.128 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: dev_1760)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.500 | 1.410 |
| High resolution limit [Å] | 1.380 | 1.380 |
| Rmerge | 0.066 | 1.124 |
| Rpim | 0.013 | 0.638 |
| Number of reflections | 23604 | |
| <I/σ(I)> | 66 | 1.5 |
| Completeness [%] | 99.2 | 85.2 |
| Redundancy | 28.4 | 6.7 |
| CC(1/2) | 1.000 | 0.842 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 50mM MES pH 6.5, 5mM Cobalt chloride, 800mM ammonium sulfate |
