4WZN
CRYSTAL STRUCTURE OF THE 2B PROTEIN SOLUBLE DOMAIN FROM HEPATITIS A VIRUS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.873 |
| Spacegroup name | P 43 |
| Unit cell lengths | 90.420, 90.420, 73.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.220 - 2.700 |
| R-factor | 0.184 |
| Rwork | 0.182 |
| R-free | 0.21300 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.130 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.536 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.099 | 0.273 |
| Number of reflections | 13240 | |
| <I/σ(I)> | 8.9 | 2.3 |
| Completeness [%] | 81.0 | 62.9 |
| Redundancy | 2.4 | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 277 | 0.1M MES PH5.2-5.8, 1.26M AMMONIUM SULFATE, 5MM DTT, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 277K |
